|1||Course Introduction; Overview of Neurotechnology||Refresh your memory of the concepts of diffraction-limited resolution, point spread function, combining tube lenses and objective lenses, and fluorescence excitation and emission at Nikon's MicroscopyU.|
Guest lecturers: Prof. Elly Nedivi, Prof. Peter So
Chen, Jerry L., Walter C. Lin, et al. "Structural Basis for the Role of Inhibition in Facilitating Adult Brain Plasticity." Nature Neuroscience 14, no. 5 (2011): 587–94.
Chen, Jerry L., Katherine L. Villa, et al. "Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex." Neuron 74, no. 2 (2012): 361–73.
So, Peter T. C., Chen Y. Dong, et al. "Two-photon Excitation Fluorescence Microscopy." Annual Review of Biomedical Engineering 2, no. 1 (2000): 399–429.
So, Peter T. C., Elijah Y. S. Yew, et al. "High-throughput Nonlinear Optical Microscopy." Biophysical Journal 105, no. 12 (2013): 2641–54.
Advanced Microscopy Imaging
Guest Lecturer: Kalen Berry, Elijah Yew
Guest lecturer: Prof. Matt Wilson
Brown, Emery N., Robert E. Kass, et al. "Multiple Neural Spike Train Data Analysis: State-of-the-art and Future Challenges." Nature Neuroscience 7, no. 5 (2004): 456–61.
Buzsáki, György, Costas A. Anastassiou, et al. "The Origin of Extracellular Fields and Currents—EEG, ECoG, LFP and Spikes." Nature Reviews Neuroscience 13, no. 6 (2012): 407–20.
Womelsdorf, Thilo, Jan-Mathijs Schoffelen, et al. "Modulation of Neuronal Interactions through Neuronal Synchronization." Science 316, no. 5831 (2007): 1609–12.
Guest Lecturer: Jon Newman
Microfluidics and Cell Engineering
Guest Lecturers: Prof. Roger Kamm & Sebastien Uzel
Kothapalli, Chandrasekhar R., Ed van Veen, et al. "A High-throughput Microfluidic Assay to Study Neurite Response to Growth Factor Gradients." Lab on a Chip 11, no. 3 (2011): 497–507.
Park, Jae Woo, Hyung Joon Kim, et al. "Advances in Microfluidics-based Experimental Methods for Neuroscience Research." Lab on a Chip 13, no. 4 (2013): 509–21.
Shin, Yoojin, Sewoon Han, et al. "Microfluidic Assay for Simultaneous Culture of Multiple Cell Types on Surfaces or within Hydrogels." Nature Protocols 7, no. 7 (2012): 1247–59.
|Lab 4||Microfluidics and Cell Engineering (cont.)|
Guest Lecturer: Dr. Daniel Berger
Lichtman, Jeff W., and Winfried Denk. "The Big and The Small: Challenges of Imaging the Brain's Circuits." Science 334, no. 6056 (2011): 618–23.
Briggman, Kevin L., and Davi D. Bock. "Volume Electron Microscopy for Neuronal Circuit Reconstruction." Current Opinion in Neurobiology 22, no. 1 (2012): 154–61.
Marx, Vivien. "Neurobiology: Brain Mapping in High Resolution." Nature 503, no. 7474 (2013): 147–52.
Tomassy, Giulio Srubek, Daniel R. Berger, et al. "Distinct Profiles of Myelin Distribution along Single Axons of Pyramidal Neurons in the Neocortex." Science 344, no. 6181 (2014): 319–24.
EM-based Connectomics (cont.)
Field Trip: Lichtman lab at Harvard
Kodandaramaiah, Suhasa B., Giovanni Talei Franzesi, et al. "Automated Whole-cell Patch-clamp Electrophysiology of Neurons in Vivo." Nature Methods 9, no. 6 (2012): 585–87.
Klapoetke, Nathan C., Yasunobu Murata, et al. "Independent Optical Excitation of Distinct Neural Populations." Nature Methods 11, no. 3 (2014): 338–46.
Chuong, Amy S., Mitra L. Miri, et al. "Noninvasive Optical Inhibition with a Red-shifted Microbial Rhodopsin." Nature Neuroscience 17, no. 8 (2014): 1123–29.
Prevedel, Robert, Young-Gyu Yoon, et al. "Simultaneous Whole-Animal 3D Imaging of Neuronal Activity Using Light-field Microscopy." Nature Methods 11, no. 7 (2014): 727–30.
|Lab 6a||Optogenetics (cont.)|
|Lab 6b||Auto Patch Clamping|
|7 & Lab||
Guest Lecturer: Dr. Ian Wickersham
Atasoy, Deniz, Yexica Aponte, et al. "A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-range Circuit Mapping." The Journal of Neuroscience 28, no. 28 (2008): 7025–30.
Wickersham, Ian R., David C. Lyon, et al. "Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically Targeted Neurons." Neuron 53, no. 5 (2007): 639–47.
Guest Lecturer: Prof. Dimitrios Pantazis
Cichy, Radoslaw Martin, Dimitrios Pantazis, et al. "Resolving Human Object Recognition in Space and Time." Nature Neuroscience 17, no. 3 (2014): 455–62.
Baillet, Sylvain, John C. Mosher, et al. "Electromagnetic Brain Mapping." (PDF) IEEE Signal Processing Magazine 18, no. 6 (2001): 14–30.
|Lab 8||MEG (cont.)|
Structural Magnetic Resonance Imaging (MRI)
Guest Lecturer: Dr. Atsushi Takahashi
Mesulam, Marsel. "The Evolving Landscape of Human Cortical Connectivity: Facts and Inferences." Neuroimage 62, no. 4 (2012): 2182–89.
|Lab 9||Structural MRI|
Functional and Molecular MRI
Guest Lecturer: Dr. Atsushi Takahashi
|Lab 10||Functional and Molecular MRI (cont.)|
High Throughput Neuroscience
Guest Lecturer: Dr. Limor Freifeld
Grewe, Benjamin F., and Fritjof Helmchen. "Optical Probing of Neuronal Ensemble Activity." Current Opinion in Neurobiology 19, no. 5 (2009): 520–29.
Looger, Loren L., and Oliver Griesbeck. "Genetically Encoded Neural Activity Indicators." Current Opinion in Neurobiology 22, no. 1 (2012): 18–23.
Lemon, William C., and Philipp J. Keller. "Live Imaging of Nervous System Development and Function using Light‐sheet Microscopy." Molecular Reproduction and Development (2013).
|Lab 11||High Throughput Neuroscience Yanik Lab|
|12||Discussion of Final Project Guidelines|
Whole-brain Optical Imaging
Guest Lecturer: Prof. Kwanghun Chung
|Chung, Kwanghun, Jenelle Wallace, et al. "Structural and Molecular Interrogation of Intact Biological Systems." Nature 497, no. 7449 (2013): 332–37.|
|14||Final Presentations I|
|15||Final Presentations II|